• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:

    公开号:SE1050402A1
    申请号:SE1050402
    申请日:2008-09-22
    公开日:2010-04-23
    发明作者:Judith N Paltiel-Zu-Cati；Robert A Starr
    申请人:Weyerhaeuser Nr Co；
    IPC主号:
    专利说明:

    In addition, the centrifuge often causes shear and compression damage to the cells.
    Thus, there is a need for a method for separating an ESM that enables reliable separation of the ESM at a relatively low cost as well as minimizing the risk of damage or contamination of the ESM.
    Summary This summary is provided for the purpose of introducing a selection of concepts in simplified form, which are further described below under the heading Detailed Description. This summary is not intended to identify the main features of the object claimed in the patent, nor is it intended to be used as an aid in determining the scope of the object claimed in the patent.
    A method for separating an embryo suspension mass is provided. The method includes applying an embryo suspension mass culture with a plurality of first particles of a first size and a number of other particles of a second size that are at least partially different from the first size of the first particles. The method also includes immersing the embryo suspension mass culture in a liquid to effect a mixture and pressing the mixture through a filter while maintaining the mixture in the liquid to separate the first particles from the second particles.
    Description of the Drawings The above aspects and several of the attendant advantages of the present invention will be more readily understood by reference to the following detailed description, taken in conjunction with the accompanying drawings.
    Figure 1 shows in perspective a filtration device constructed according to an embodiment of the present invention.
    Figure 2 shows a cross-sectional view from the side of a manufactured seed.
    Figure 3 is an exploded perspective view of the filtration device of Figure 1.
    Figure 4 is a perspective view of a cross-sectional view of a filtration device according to claim 1. Detailed Description A filtration device 120 constructed according to an embodiment of the present invention is best seen in Figure 1. The filtration device 120 may be suitably designed for inclusion. in an automated unit used for growing embryos for use in the production of artificial seeds.
    Alternatively, such a filtering device 120 is suitably adapted to act alone as a separate unit for harvesting either embryos or culture used for culturing embryos for insertion into well-known manufactured seeds.
    For explanatory purposes and as a background, the structure of a manufactured seed 20 is described with reference to Figure 2. The manufactured seed 20 includes a cylindrical lid 22 ("cylcap"), a seed coat 24, a nutrient medium 26, such as a gametophyte, and a bottom closure 28. The seed coat 24 is suitably formed of a section of tubular material. In one embodiment, the seed coat 24 is a section of a straw of fibrous material, such as paper. The sections of the suction tube may be pretreated in a suitable coating material, such as wax.
    The cylindrical lid 22, also known as a limiter, is suitably made from a porous material having a hardness strong enough to withstand puncture or fracture caused by a germinating embryo, such as a ceramic or porcelain material, and includes an end cap. closure member 30 and a heart blade restraining member 32. The heart blade restraining member 32 is suitably integrally or uniformly shaped together with the end closure member 30. The cylindrical cover 22 also includes a longitudinally extending cavity 34 extending through the end closure member 30 and partially through one end of the heart blade restraint member 32. .
    The cavity 34 is sized to receive a plant embryo 42 therein.
    In certain embodiments, such as when the cylindrical lid 22 is suitably made of a porous material, it may be desirable to coat the cylindrical lid 22 with a barrier material to reduce the degree of water loss and to limit or reduce microbial entry. Such barriers include wax, polyurethane, glaze, nail polish, and a coating sold by Airproducts Airflex 4514. The embryo 42 is disposed within the cavity 34 and is suitably enclosed therein by means of a living end closure 43. The living end closure 43 includes a primary end closure 44 and a secondary end closure 21. The primary end closure 44 is suitably formed of a PCL material, as described above, and includes a centrally located opening 50. The opening 50 is sized to accommodate it corresponds to the diameter of the cavity 34 of the cylindrical lid 22 to allow a germinating embryo 42 to pass therethrough. The primary end closure 44 may conveniently be attached to the end closure member 30 by a variety of methods, including adhesive or heat bonding. Finally, the manufactured seed 20 includes a bottom closure 28 and may include a tertiary closure 60.
    As best seen by reference to Figures 3 and 4, the filtration device 120 includes a cup 122, a rotating assembly 124 and a basket 126. A well known aerator 128 is attached to the cup 122. Such a aerator 128 includes a vent tube 130, a lid 132 and a clamp 134. The aerator 128 is suitably arranged inside the cup 122 and, as is well known, aerates the contents of the cup 122.
    The rotating assembly 124 includes a feed tube 140, a spindle sleeve 142, a magnet 144 and a pin 146. The feed tube 140 is "broken" for illustrative purposes and includes an inlet port 150 extending through a side wall of the tube 140. One end of the feed tube 140 defines a feed end 152 while the other end defines an anchor end 154. The anchor end 154 is sized to slidably receive the pin 146 therein.
    The pin 146 is suitably a cylindrically shaped element and includes a spherically shaped tip 160 extending from one end of the pin 146.
    The other end of the pin 146 includes a shoulder 162. The tip 160 is preferably spherically shaped to minimize damage to particulate matter, such as embryo suspension mass ("ES1 / 1"), which is supplied through the inlet end 152 to the feed tube 140, as described in more detail below. .
    After the pin 146 is inserted into the anchor end 154 of the feed tube 140, a screw 148 is inserted into a corresponding opening (not shown) formed in the shoulder 162 of the pin 146. The insertion of the screw 148 into the opening of the pin 146 causes the end of the pin 146 to expand into the anchoring end 154 of the feed tube 140, which secures the pin 146 to the feed tube 140 by compression fitting. Thereafter, the magnet 144 is inserted through a channel 164 extending through one end of the spindle sleeve 142.
    The rotating assembly 124 is attached to the basket 126 via an impeller 170. The impeller 170 includes an externally threaded shaft 172 which is sized to engage in a threaded manner with corresponding internal threads in the spindle sleeve 142. In the connected condition, the impeller 170 rotates with the rotating the assembly 124 by means of a well-known magnetic drive assembly (not shown), which is suitably located below the cup 122. The feed end 152 of the feed pipe 140 extends through the basket 126 and through a cup lid 180 and is closed thereto by means of an end cap 182. and an O-ring 184.
    As best seen in Figure 4, the basket 126 includes a plurality of pores 190 that are sized to filter the ESM. More specifically, the ESM includes a plurality of first particles of a first size and a plurality of second particles of a second size. As is known in the art, ESM is a population of variable size clusters and associations of single embryonic cells with suspender cells and individual suspender cells and cellular debris. Thus, the term "particle" as used herein is intended to include single embryonic cells, multiple embryonic cells, suspender cells, cellular debris, embryos, etc., and any combination thereof.
    In operation of the filtering device 120, the ESM is filtered through the basket 126 to segregate the plurality of particles constituting the ESM. The pores 190 may, for example, have a diameter extending between 0.25 mm and 1.5 mm. The pores 190 may be 0.5 mm, 0.75 mm, 1.00 mm or 1.25 mm in diameter as non-limiting examples. In a non-limiting example of a basket 126 with pores 190 with a diameter of 0.5 mm, the ESM filtered through the basket 126 allows particles with a diameter of about 0.5 mm and smaller to pass through the pores 190. The basket 126 filters as such at least particles with a diameter of about 0.5 mm from particles with a diameter of more than 0.5 mm. The operation of the filtering device 120 is most readily understood by reference to Figures 1 and 4. An input of ESM is provided to the input tube 140 via the input end 152. The ESM material fl flows into the input tube 140, from which it is fed into the basket 126 through the inlet opening 150. The basket 126 is located in the cup 122, which is filled with a suspension medium, such as a liquid. As noted above, the flow of ESM is attenuated to limit damage to particulate matter in the ESM by means of the spherical end 160.
    The ESM suspended in the liquid in the cup 122 creates a mixture which is forced through the filter or basket 126 by means of, for example, centrifugal force.
    When the mixture is forced through the pores 190 in the basket 126, a plurality of first particles of a first size are separated from a plurality of second particles of a second size that are at least partially different from the first size of the first particles. For example, if the first particles are cells of larger, more mature embryos and the second particles are smaller in size than the more mature cells, the smaller cells pass through the pores 190 if they are smaller than the diameter of the openings 190 and into the bottom of the beaker. 122. For example, if the basket 126 is magnetically driven to induce a rotational force, the centrifugal force of the rotating basket 126 drives the other particles through the pores 190. Although illustrative embodiments have been illustrated and described, it will be appreciated that various changes may be made therefrom. to depart from the spirit and scope of the invention. As a non-limiting example, the filtering device 120 may include a second basket or filtering layer where the second basket includes pores of a different diameter compared to the first basket. In such an embodiment, the filtration devices provide two filtration layers for segregation of particles from the ESM material into separate steps. Although two baskets are described, it is apparent that several baskets may be included to provide as many filtration layers as desired.
    权利要求:
    Claims (9)
    [1]
    A method of separating an embryo suspension mass, the method comprising: (a) providing an embryo suspension mass culture having a plurality of first particles having a first size and a plurality of second particles having a second size that is at least partially different from the first size; the play of the first particles; (b) immersing the embryo suspension mass culture in a liquid to produce a mixture; and (c) pressing the mixture through at least a first filter while maintaining the mixture in the liquid to separate the first particles from the second particles.
    [2]
    The method of claim 1, wherein pressing the mixture through at least a first filter includes applying a centrifugal force to the mixture while maintaining the mixture in the liquid.
    [3]
    The method of claim 1, wherein the immersion of the embryo suspension mass culture comprises depositing the embryo suspension mass culture in a basket with a plurality of pores sized to allow at least the second particles, but not the first particles, to pass through the plurality of pores.
    [4]
    The method of claim 3, wherein pressing the mixture through at least one first filter includes applying a rotary motion to the basket to press at least the second particles through the plurality of pores while retaining at least the first particles in the basket.
    [5]
    A method of separating an embryo suspension mass, the method comprising: (a) providing an embryo suspension mass culture having a plurality of first particles of a first size and a number of second particles having a b 10 (20) 30 b) (C) 8 a second size which is at least partially different from the first size of the first particles; immersing the embryo suspension mass culture in a liquid to produce a mixture; and applying a rotary motion to the mixture to press the mixture through a filter to separate the first particles from the second particles.
    [6]
    The method of claim 5, wherein the application of a rotary motion to the mixture includes applying a centrifugal force to a filter holding the mixture.
    [7]
    The method of claim 5, wherein the filter is a basket of a plurality of pores sized to filter at least the first particles from the second particles.
    [8]
    The method of claim 7, wherein applying a rotary motion to the mixture includes applying a centrifugal force to the basket.
    [9]
    A method of separating an embryo suspension mass, the method comprising: (a) b) (C) providing an embryo suspension mass culture having a plurality of first particles having a first size and a plurality of second particles having a second size that is at least partially different from the first size of the first particles; depositing the embryo suspension mass culture in a basket with a plurality of pores sized to filter at least the first particles from the second particles; immersing the basket containing the embryo suspension mass culture in a liquid to produce a mixture; and applying a rotary motion to the mixture to press the mixture through the plurality of pore baskets to separate the first particles from the second particles.
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    IL88266A|1987-11-18|1998-03-10|Phytogen|Method for the regeneration of a cotton plant from somatic cotton cells|
    EP0934691A3|1993-01-15|2001-03-28|Novartis Seeds B.V.|Improvements in or relating to embryogenic compounds|
    US8039260B2|2004-01-08|2011-10-18|Dow Agrosciences Llc|Chlorophyllous totipotent maize cell cultures|AR089280A1|2011-12-29|2014-08-13|Weyerhaeuser Nr Co|AUTOMATIC SYSTEM AND METHODS TO SEPARATE AND ISOLATE EMBRYOS FROM PLANTS|
    JP6444314B2|2013-01-11|2018-12-26|リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ．|System and device for sample handling|
    JP2018526992A|2015-08-31|2018-09-20|アイ ピース，インコーポレイテッド|Pluripotent stem cell production system, stem cell induction method, stem cell suspension culture method, stem cell suspension incubator, method for producing induced pluripotent stem cells, and method for producing specific somatic cells from animal cells|
    法律状态:
    2018-05-02| NUG| Patent has lapsed|
    优先权:
    申请号 | 申请日 | 专利标题
    US97525007P| true| 2007-09-26|2007-09-26|
    PCT/US2008/077175|WO2009042536A1|2007-09-26|2008-09-22|Method of separating embryo suspension mass|
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